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  1. #1
    Senior Member jp_48504's Avatar
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    Apr 2005

    Production Of Phenylalanine In Aspartame Through E. coli

    Production Of Phenylalanine In Aspartame Through E. coli.

    By Dr. Betty Martini, D.Hum.

    The information keeps being exposed that aspartame is genetically engineered. Today this article discusses trial and error. In this past quarter of a century this genetically engineered, addictive, excitoneurotoxic carcinogenic drug has caused the death of millions . The medical text, Aspartame Disease: An Ignored Epidemic, by H. J. Roberts, M.D., is 1038 pages of symptoms, diseases, tumors and other horrors triggered by this deadly chemical poison. What is E. coli? You find it in the intestines. Here is a description:

    Think back years ago during the aspartame wars and congressional hearings when physicians and scientists were outraged by the propaganda of the manufacturer about how natural aspartame is like bananas or milk. They knew all along aspartame is toxic sludge. Aspartame is not natural and was not made by Jehovah God. Aspartame is another genetically engineered poison.

    To make matters worse the front groups like Calorie Control Council and professional organizations continually tell pregnant women its safe to use even though they know its an abortifacient and teratogen causing birth defects and mental retardation. They know the depletion of serotonin is triggering psychiatric and behavioral problems, and that aspartame interacts with all drugs and vaccines. Our children don't have a chance. For this reason this year we have been emailing to the schools, pediatricians and OB-GYN new reports by the experts - Report to Schools: ... ildren.htm

    Ajinomoto actually took out a full page ad, published in the Nov 2004 edition of "Functional Foods and Nutraceuticals" saying "Remember your first taste of aspartame" showing a baby nursing at its mother's breast. In the Report to Schools you will see Dr. Roberts paper that discusses the baby can get aspartame through mother's milk if she is using it. Dr. Roberts and I reported them to the FCC.

    Now the FDA has approved spraying our food with viruses. How much more can they do to be responsible for unsafe food and drugs?

    Dr. Betty Martini, D.Hum, Founder
    Mission Possible International
    9270 River Club Parkway
    Duluth, Georgia 30097
    770 242-2599 and

    Taking The Guesswork Out Of Microbial Production
    By Ahmed ElAmin

    A Netherlands-based research company claims a breakthrough in developing a more efficient way to produce the microorganisms used in industrial food production.

    The process brings cost-efficient and sustainable microbial production within reach of all companies and reduces the time-to-market for new bioprocesses, ingredients, functional products and amino acids, says TNO, a contract research company based in the country. The process can also be used to produce the microbial strains used by the food industry to extend shelf life, kill pathogens and to improve quality.

    Microorganisms play an important role in the industrial production of food and feed ingredients, medicines and other compounds. In fact, using microorganisms to produce foods such as yoghurt, beer, cheese has been around a long time.

    One way to achieve commercially viable production levels is through genetic strain improvement, a key aspect in industrial biotechnology. Since microorganisms can contain over five thousands genes, about half of which have a function that is still unknown, processors generally used a trial-and-error method.

    This can waste a company's time and money. Current methods to improve conventional microbial production processes have so far resulted in efficiency gains of only one to three per cent, the company said in an announcement today.

    "The industry is continuously looking for ways to increase the efficiency of microbial production processes," TNO stated in a press release. " However, to a large extend, the bacterial cell metabolism is still a black box. Therefore, until now, process optimisation has been mainly a process of trial-and-error, resulting in only small improvements for large scale processes."

    The TNO process, which the company developed over the past five years, can boost microbal production yield by 50 per cent in nine months, the company claimed in an announcement today.

    One way to achieve commercially viable production levels is through genetic strain improvement, which the TNO process attempts to address. The process applies to the genetic improvement of microbial production strains that play a role in the fermentation or enzymatic conversion of biomaterials into end products.

    The core of TNO's process is its metabolomics toolbox. Metabolomics is the study of specific cellular processes and the end products. TNO said its process allowed it to process large amounts of data on microorganisms, compile it, then create a systematic method for harnessing those processes.

    Metabolomics is a genomics technology that measures and compares all metabolites - intermediates of chemical reactions - that are present in a microbial cell. Like other genomics technologies, the research generates an avalanche of data. TNO was able to take the data, extract the relevant information, and identify methods for genetically improving the microbial production strains used in food production.

    The data was collected in the toolbox, which the company is marketing as a product to the food industry.

    "TNO has been able to identify the targets that are most relevant for improving the production process, without any bias, thus accelerating process optimisation," the company stated. "In addition, application of the toolbox has resulted in new insights into cellular metabolism, generating new intellectual property opportunities."

    TNO said it has successfully used its new metabolomics toolbox for several applications. One example is the production of phenylalanine, a building block for the artificial sweetener aspartame, through the use of the bacterium E. coli. It is also a precursor in the production of some aromatic compounds and antibiotics.

    TNO said it was able to use the toolbox to increase phenylalanine production yield by 50 per cent.

    Microbials are increasingly being used to produce "pure ingredients", such as single vitamins and flavours, said Mariët van der Werf, TNO's product manager for microbial production processes in Zeist, the Netherlands.

    "The technology is still in its early stages," she told in an interview. "Our technology has broad applications in the food industry." ... -guesswork
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  2. #2

    Join Date
    Jan 1970
    Outside the P.R.of Boulder
    The information keeps being exposed that aspartame is genetically engineered.
    Oh for Pete's sake... Aspartame is NOT genetically engineered. It can not be since the chemical is not alive. At most one can say that it is molecularly engineered, but then the number of OTHER chemicals in our foodstocks and environment is legion.

    As for producing phenylalanine in E. colo, KEWL! Lots of our foodstuffs are produced using bacteria of some species or another. You've 'prolly eaten some today.

    Worried about the possible health effects of aspartame? Then don't consume the stuff. There is no big secret being kept about it, data on the substance was available 20 some years ago in any decent University library, when I chose to rarely use the stuff.
    Knowledge is Power Power corrupts Study hard Be Evil

  3. #3
    Senior Member jp_48504's Avatar
    Join Date
    Apr 2005
    My choice has been to stay away from all processed food.
    I stay current on Americans for Legal Immigration PAC's fight to Secure Our Border and Send Illegals Home via E-mail Alerts (CLICK HERE TO SIGN UP)

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